4th April 2019: IGV Genome Browser
The Broad Institute have changed where they host the genome files for IGV (from igvdata.broadinstitute.org/* to https://s3.amazonaws.com/igvdata.broadinstitute.org/*). As the latter is not yet 'white-listed' within the Research Environment, the genome files will not be accessible causing an error to occur when attempting to load them within IGV.
We have manually downloaded the FASTA, RefSeq annotation, chromosome cytoband, and chromosome aliases from the Broad for hg19/GRCh37 and hg38/GRCh38 reference assemblies and have created .genome files from them. These are identical to the default ones present in IGV and can be loaded into IGV using the instructions below.
|1||Open the IGV application from the desktop||-|
|2||Click 'Cancel' on the subsequent pop-up box|
|3||On the IGV toolbar, navigate to 'Genomes' > 'Load Genome from File...'|
|4||In the file browser, navigate to 'Home' > 'public_data_resources'|
|5||Navigate to 'IGV'|
|6||Select the reference assembly of choice (hg19/GRCh37 or hg38/GRCh38).|
|7||Click on the corresponding '.genome' file.|
|8||This will load the genome file selected as a local version|